Rapid lysostaphin test to differentiate Staphylococcus and Micrococcus species

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Rapid lysostaphin test to differentiate Staphylococcus and Micrococcus species.

A rapid, simple lysostaphin lysis susceptibility test to differentiate the genera Staphylococcus and Micrococcus was evaluated. Of 181 strains from culture collections, 95 of 95 Staphylococcus strains were lysed, and 79 of 79 Micrococcus strains were not lysed. The seven Planococcus strains were resistant. Clinical isolates (890) were tested with lysostaphin and for the ability to produce acid ...

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Rapid identification of Staphylococcus aureus by using lysostaphin sensitivity.

Lysostaphin sensitivity was evaluated as a rapid screening test to differentiate Staphylococcus aureus from other species of staphylococci and micrococci. A total of 168 strains of staphylococci, 108 of which were S. aureus, were cultured overnight in brain infusion broth. Gram stains were peformed before and after a 1:10 dilution of the culture was exposed to 2 micrograms of lysostaphin per ml...

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Variations in bacitracin susceptibility observed in Staphylococcus and Micrococcus species.

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Susceptibility of methicillin-resistant Staphylococcus aureus to lysostaphin.

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Staphylococcus aureus FDA 209P cells when suspended in 24% (w/v) NaCl were rendered osmotically fragile by exposure to lysostaphin for time intervals ranging from 2 to 60 min. Such cells were analyzed chemically and serologically for evidence of residual cell wall material, were plated in hypertonic sucrose medium to determine revertibility to normal, and were subjected to manometric studies to...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1986

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.23.6.1044-1045.1986